Ahmad, 12 hours is not a ...

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Ahmad,

12 hours is not a large time gap. However, in order to maintain levels, you can have a pump based recycle of the wastewater (capacity of pump should be such that it should be able to keep the contents stirring slowly, not vigourously) inside the tank to keep the contents agitated, and circulating. The surface oxygen exchange will suffice.

Dont worry. Having a 12 hour downtime doesnt kill the bacteria, it just starves them. Once you restart the process, just make sure to add a bit of additional culture, urea and dap.